algAI

The Burden of LGMDR1

Ab-silica-LNP Architecture

We are developing an Ab-silica-LNP (lipid nanoparticle) for the delivery of a musculoskeletal cell-targeting gene therapy payload consisting of mRNA that encodes gRNA and Caspase 9 (or other) to base-edit via Non-Homologous End Joining (NHEJ) the CAPN3 gene in LGMDR1 patients.

Here, a diatom microalgae supplies the 3-D silica core and 3 red microalgae supply the single-domain anti-CD71 nanobody, transferrin, and Myomaker fusogen proteins for specific targeting. The lipids used are comprised of an optimized mixture of cholesterol, helper, cationic, ionizable, and membrane diffusing-lipids, as well as stealth PEG-like polymers to avoid immunogenicity.

Colorectal Cancer Theranostics

We utilize an albumin-Ab-BPE-FeCl3-silica-LNP for the delivery of an orally ingested, gastrointestinally-delivered payload consisting of magnetic FeCl3 particles, albumin-fused anti-EGFR targeting nanobody, and red fluorescent B-phycoerythrin (B-PE) to target and destroy colorectal cancer tumors.

Here, a diatom microalgae that is cultivated on iron-enriched growth media supplies the magnetic 3-D FeCl3 silica core, and a red microalgae supplies the immunoconjugate of albumin-fused, B-PE fused anti-EGFR targeting nanobody.

Photo-Dynamic & Photo-Thermal Therapy

This B-PE-anti-EGFR immunoconjugate can alone be sold as a diagnostic reagent for flow cytometric applications. However, we instead affix the albumin-B-PE-anti-EGFR to the silica-LNP as an endothelial cell-targeting ligand to deliver a payload of red-light activated B-PE fluorescence-based photo-dynamic therapy (destruction of tumor via free oxygen radical formation in relatively hypoxic tumor environment) and a UV-light and external magnetic signal-activated FeCl3-based photo-thermal therapy (destruction of tumor via heat generated). This approach completely eliminates the off-targeting risk of standard chemotherapy.

Hydrocolloids & Co-Products Processing

Another strain of red microalgae whose metabolic pathway has not been knocked out will naturally secrete a uniquely thermo-and acid-gelling, linear, anionic, polysaccharide (LASP) that is then used to formulate a pH-responsive hydrogel capsule that houses the albumin-Ab-BPE-FeCl3-silica-LNP for convenient and cheaper oral delivery.

The red B-PE protein co-product, with or without protein engineering and pH-Temperature stabilizing mutagenesis, can be used for myriad applications (i.e. food and beverage, cosmetics, textiles, medical diagnostics). Similarly, the LASP polysaccharide can be used for myriad applications (i.e. as replacement for xanthan or guar gums for oil and gas extraction, as a thermo-gelling agent for plant-based meat applications, as a cosmetic ingredient).

High-Yield Neutral Lipids

We harvest neutral lipids (i.e. tri-acyl-glycerides) for the production of sustainable jet fuel (SAF) for the civilian and military aviation markets and DOE research grant opportunities, as well as biodiesel for the back-up generators that all AI data centers (currently a $3 trillion construction project) rely on.

Here, a large, multi-trophic, genetically-amenable, filamentous microalgae that can be cultivated outdoors in large and cheap raceway open-ponds with minimal contamination-crash risk and be harvested without the need for expensive ultrafiltration or centrifugation or dissolved air flotation supplies lipid bodies in its cytoplasm.

Battery & Electrolyte Materials

Cellulose that is extracted from the aforementioned filamentous algae's easy-to-lyse wall is converted to a nano-cellulose co-product which has myriad applications.

For example, it can be combined with the silica of the aforementioned diatom microalgae to produce an anodic or cathodic binder and electrolyte material for batteries for electric vehicles (EVs) and storing overnight excess power generated for the massive data centers currently being built out.